| Spire Alexandra Hospital | Chatham | Kent |
| Spire Clare Park Hospital | Farnham | Surrey |
| Spire Gatwick Park Hospital | Horley | Surrey |
| Spire Hartswood Hospital | Brentwood | Essex |
| Spire Regency Hospital | Macclesfield | Cheshire |
| Spire Shawfair Park Hospital | Edinburgh | |
| Spire South Bank Hospital | Worcester | Worcestershire |
| Spire Thames Valley Hospital | Slough | Buckinghamshire |
| Spire Bristol Hospital | Bristol | Avon |
| Spire Bushey Hospital | Watford | Hertfordshire |
| Spire Cambridge Lea Hospital | Cambridge | Cambridgeshire |
| Spire Cardiff Hospital | Cardiff | South Glamorgan |
| Spire Elland Hospital | Elland | West Yorkshire |
| Spire Fylde Coast Hospital | Blackpool | Lancashire |
| Spire Harpenden Hospital | Harpenden | Hertfordshire |
| Spire Hull and East Riding Hospital | Anlaby | East Yorkshire |
| Spire Leeds Hospital | Leeds | West Yorkshire |
| Spire Leicester Hospital | Leicester | Leicestershire |
| Spire Little Aston Hospital | Sutton Coldfield | West Midlands |
| Spire Liverpool Hospital | Liverpool | |
| Spire Manchester Hospital | Manchester | Greater Manchester |
| Spire Murrayfield Hospital, Edinburgh | Edinburgh | Scotland |
| Spire Murrayfield Hospital, Wirral | Wirral | Merseyside |
| Spire Norwich Hospital | Norwich | Norfolk |
| Spire Parkway Hospital | Solihull | West Midlands |
| Spire Portsmouth Hospital | Havant | Hampshire |
| Spire Roding Hospital | Redbridge | Essex |
| Spire Southampton Hospital | Southampton | Hampshire |
| Spire St Saviour's Hospital | Hythe | Kent |
| Spire Tunbridge Wells Hospital | Tunbridge Wells | Kent |
| Spire Washington Hospital | Washington | Tyne and Wear |
| Spire Wellesley Hospital | Southend-on-Sea | Essex |
| Spire Yale Hospital | Wrexham | |
